Impact of next-generation sequencing error on analysis of barcoded plasmid libraries of known complexity and sequence.

ABSTRACT: Barcoded vectors are promising tools for investigating clonal diversity and dynamics in hematopoietic gene therapy. Analysis of clones marked with barcoded vectors requires accurate identification of potentially large numbers of individually rare barcodes, when the exact number, sequence identity and abundance are unknown. This is an inherently challenging application, and the feasibility of using contemporary next-generation sequencing technologies is unresolved. To explore this potential application empirically, without prior assumptions, we sequenced barcode libraries of known complexity. Libraries containing 1, 10 and 100 Sanger-sequenced barcodes were sequenced using an Illumina platform, with a 100-barcode library also sequenced using a SOLiD platform. Libraries containing 1 and 10 b

SUBMITTER: Deakin CT 

PROVIDER: S-EPMC4176369 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature