Development of a forward genetic screen to isolate oil mutants in the green microalga Chlamydomonas reinhardtii.

ABSTRACT:

Background

Oils produced by microalgae are precursors to biodiesel. To achieve a profitable production of biodiesel from microalgae, identification of factors governing oil synthesis and turnover is desirable. The green microalga Chlamydomonas reinhardtii is amenable to genetic analyses and has recently emerged as a model to study oil metabolism. However, a detailed method to isolate various types of oil mutants that is adapted to Chlamydomonas has not been reported.

Results

We describe here a forward genetic approach to isolate mutants altered in oil synthesis and turnover from C. reinhardtii. It consists of a three-step screening procedure: a primary screen by flow cytometry of Nile red stained transformants grown in 96-deep-well plates under three sequential conditions (p

SUBMITTER: Cagnon C 

PROVIDER: S-EPMC4176504 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature