Different nucleosomal architectures at early and late replicating origins in Saccharomyces cerevisiae.

ABSTRACT:

Background

Eukaryotic genomes are replicated during S phase according to a temporal program. Several determinants control the timing of origin firing, including the chromatin environment and epigenetic modifications. However, how chromatin structure influences the timing of the activation of specific origins is still poorly understood.

Results

By performing high-resolution analysis of genome-wide nucleosome positioning we have identified different chromatin architectures at early and late replication origins. These different patterns are already established in G1 and are tightly correlated with the organization of adjacent transcription units. Moreover, specific early and late nucleosomal patterns are fixed robustly, even in rpd3 mutants in which histone acetylation and orig

SUBMITTER: Soriano I 

PROVIDER: S-EPMC4176565 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature