Antigen-specific, antibody-coated, exosome-like nanovesicles deliver suppressor T-cell microRNA-150 to effector T cells to inhibit contact sensitivity.

ABSTRACT:

Background

T-cell tolerance of allergic cutaneous contact sensitivity (CS) induced in mice by high doses of reactive hapten is mediated by suppressor cells that release antigen-specific suppressive nanovesicles.

Objective

We sought to determine the mechanism or mechanisms of immune suppression mediated by the nanovesicles.

Methods

T-cell tolerance was induced by means of intravenous injection of hapten conjugated to self-antigens of syngeneic erythrocytes and subsequent contact immunization with the same hapten. Lymph node and spleen cells from tolerized or control donors were harvested and cultured to produce a supernatant containing suppressive nanovesicles that were isolated from the tolerized mice for testing in active and adoptive cell-transfer models of CS.

R

SUBMITTER: Bryniarski K 

PROVIDER: S-EPMC4176620 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature