Quantification of Al₂O₃ nanoparticles in human cell lines applying inductively coupled plasma mass spectrometry (neb-ICP-MS, LA-ICP-MS) and flow cytometry-based methods.

ABSTRACT: In order to quantify and compare the uptake of aluminum oxide nanoparticles of three different sizes into two human cell lines (skin keratinocytes (HaCaT) and lung epithelial cells (A549)), three analytical methods were applied: digestion followed by nebulization inductively coupled plasma mass spectrometry (neb-ICP-MS), direct laser ablation ICP-MS (LA-ICP-MS), and flow cytometry. Light and electron microscopy revealed an accumulation and agglomeration of all particle types within the cell cytoplasm, whereas no particles were detected in the cell nuclei. The internalized Al₂O₃ particles exerted no toxicity in the two cell lines after 24 h of exposure. The smallest particles with a primary particle size (x_BET) of 14 nm (Alu1) showed the lowest sedime

SUBMITTER: Bohme S 

PROVIDER: S-EPMC4176630 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature