On-chip magnetic separation and encapsulation of cells in droplets.

ABSTRACT: Single cell study is gaining importance because of the cell-to-cell variation that exists within cell population, even after significant initial sorting. Analysis of such variation at the gene expression level could impact single cell functional genomics, cancer, stem-cell research, and drug screening. The on-chip monitoring of individual cells in an isolated environment would prevent cross-contamination, provide high recovery yield, and enable study of biological traits at a single cell level. These advantages of on-chip biological experiments is a significant improvement for a myriad of cell analyses methods, compared to conventional methods, which require bulk samples and provide only averaged information on cell structure and function. We report on a device that integrates a mobile mag

SUBMITTER: Chen A 

PROVIDER: S-EPMC4176703 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature