Osteopontin is an important mediator of alcoholic liver disease via hepatic stellate cell activation.

ABSTRACT:

Aim

To investigate over-expression of Osteopontin (OPN) pathway expression and mechanisms of action in human alcoholic liver disease (ALD), in vivo and in vitro acute alcohol models.

Methods

OPN pathway was evaluated in livers from patients with progressive stages of human ALD and serum from drinkers with and without liver cirrhosis. In vitro stellate LX2 cells exposed to acute alcohol and in vivo in acute alcoholic steatosis mouse models were also investigated for OPN pathway expression and function. WT and OPN(-/-) mice were administered an acute dose of alcohol and extent of liver injury was examined by histopathology and liver biochemistry after 16-24 h. The causative role of OPN was studied in OPN knockout animals and in vitro in stellate LX2 cells, utilizing siRNA, apt

SUBMITTER: Seth D 

PROVIDER: S-EPMC4177488 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature