ECMIS: computational approach for the identification of hotspots at protein-protein interfaces.

ABSTRACT:

Background

Various methods have been developed to computationally predict hotspot residues at novel protein-protein interfaces. However, there are various challenges in obtaining accurate prediction. We have developed a novel method which uses different aspects of protein structure and sequence space at residue level to highlight interface residues crucial for the protein-protein complex formation.

Results

ECMIS (Energetic Conservation Mass Index and Spatial Clustering) algorithm was able to outperform existing hotspot identification methods. It was able to achieve around 80% accuracy with incredible increase in sensitivity and outperforms other existing methods. This method is even sensitive towards the hotspot residues contributing only small-scale hydrophobic interactions

SUBMITTER: Shingate P 

PROVIDER: S-EPMC4177600 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature