Elevation of extracellular Ca2+ induces store-operated calcium entry via calcium-sensing receptors: a pathway contributes to the proliferation of osteoblasts.

ABSTRACT:

Aims

The local concentration of extracellular Ca(2+) ([Ca(2+)]o) in bone microenvironment is accumulated during bone remodeling. In the present study we investigated whether elevating [Ca(2+)]o induced store-operated calcium entry (SOCE) in primary rat calvarial osteoblasts and further examined the contribution of elevating [Ca(2+)]o to osteoblastic proliferation.

Methods

Cytosolic Ca(2+) concentration ([Ca(2+)]c) of primary cultured rat osteoblasts was detected by fluorescence imaging using calcium-sensitive probe fura-2/AM. Osteoblastic proliferation was estimated by cell counting, MTS assay and ATP assay. Agonists and antagonists of calcium-sensing receptors (CaSR) as well as inhibitors of phospholipase C (PLC), SOCE and voltage-gated calcium (Cav) channels were applied t

SUBMITTER: Hu F 

PROVIDER: S-EPMC4177836 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature