Generalized unmixing model for multispectral flow cytometry utilizing nonsquare compensation matrices.

ABSTRACT: Multispectral and hyperspectral flow cytometry (FC) instruments allow measurement of fluorescence or Raman spectra from single cells in flow. As with conventional FC, spectral overlap results in the measured signal in any given detector being a mixture of signals from multiple labels present in the analyzed cells. In contrast to traditional polychromatic FC, these devices utilize a number of detectors (or channels in multispectral detector arrays) that is larger than the number of labels, and no particular detector is a priori dedicated to the measurement of any particular label. This data-acquisition modality requires a rigorous study and understanding of signal formation as well as unmixing procedures that are employed to estimate labels abundance. The simplest extension of the tradition

SUBMITTER: Novo D 

PROVIDER: S-EPMC4177998 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature