A toolbox for spatiotemporal analysis of voltage-sensitive dye imaging data in brain slices.

ABSTRACT: Voltage-sensitive dye imaging (VSDI) can simultaneously monitor the spatiotemporal electrical dynamics of thousands of neurons and is often used to identify functional differences in models of neurological disease. While the chief advantage of VSDI is the ability to record spatiotemporal activity, there are no tools available to visualize and statistically compare activity across the full spatiotemporal range of the VSDI dataset. Investigators commonly analyze only a subset of the data, and a majority of the dataset is routinely excluded from analysis. We have developed a software toolbox that simplifies visual inspection of VSDI data, and permits unaided statistical comparison across spatial and temporal dimensions. First, the three-dimensional VSDI dataset (x,y,time) is geometrically tra

SUBMITTER: Bourgeois EB 

PROVIDER: S-EPMC4178182 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature