Ets-1 global gene expression profile reveals associations with metabolism and oxidative stress in ovarian and breast cancers.

ABSTRACT:

Background

The Ets-1 proto-oncogene is frequently upregulated in cancer cells, with known involvement in cancer angiogenesis, metastasis, and more recently energy metabolism. In this study we have performed various bioinformatic analyses on existing microarray data to further clarify the role of Ets-1 in ovarian cancer, and validated these results with functional assays.

Methods

Functional pathway analyses were conducted on existing microarray data comparing 2008 and 2008-Ets1 ovarian cancer cells. Methods included over-representation analysis, functional class scoring and pathway topology, and network representations were visualized in Cytoscape. Oxidative stress regulation was examined in ovarian cancer cells by measuring protein expression and enzyme activity of glutathio

SUBMITTER: Verschoor ML 

PROVIDER: S-EPMC4178218 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature