Decoration of outer membrane vesicles with multiple antigens by using an autotransporter approach.

ABSTRACT: Outer membrane vesicles (OMVs) are spherical nanoparticles that naturally shed from Gram-negative bacteria. They are rich in immunostimulatory proteins and lipopolysaccharide but do not replicate, which increases their safety profile and renders them attractive vaccine vectors. By packaging foreign polypeptides in OMVs, specific immune responses can be raised toward heterologous antigens in the context of an intrinsic adjuvant. Antigens exposed at the vesicle surface have been suggested to elicit protection superior to that from antigens concealed inside OMVs, but hitherto robust methods for targeting heterologous proteins to the OMV surface have been lacking. We have exploited our previously developed hemoglobin protease (Hbp) autotransporter platform for display of heterologous polypepti

SUBMITTER: Daleke-Schermerhorn MH 

PROVIDER: S-EPMC4178611 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature