Transcriptome analysis of the entomopathogenic oomycete Lagenidium giganteum reveals putative virulence factors.

ABSTRACT: A combination of 454 pyrosequencing and Sanger sequencing was used to sample and characterize the transcriptome of the entomopathogenic oomycete Lagenidium giganteum. More than 50,000 high-throughput reads were annotated through homology searches. Several selected reads served as seeds for the amplification and sequencing of full-length transcripts. Phylogenetic analyses inferred from full-length cellulose synthase alignments revealed that L giganteum is nested within the peronosporalean galaxy and as such appears to have evolved from a phytopathogenic ancestor. In agreement with the phylogeny reconstructions, full-length L. giganteum oomycete effector orthologs, corresponding to the cellulose-binding elicitor lectin (CBEL), crinkler (CRN), and elicitin proteins, were characterized by doma

SUBMITTER: Quiroz Velasquez PF 

PROVIDER: S-EPMC4178636 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature