Combinatorial strategies for improving multiple-stress resistance in industrially relevant Escherichia coli strains.

ABSTRACT: High-cell-density fermentation for industrial production of chemicals can impose numerous stresses on cells due to high substrate, product, and by-product concentrations; high osmolarity; reactive oxygen species; and elevated temperatures. There is a need to develop platform strains of industrial microorganisms that are more tolerant toward these typical processing conditions. In this study, the growth of six industrially relevant strains of Escherichia coli was characterized under eight stress conditions representative of fed-batch fermentation, and strains W and BL21(DE3) were selected as platforms for transposon (Tn) mutagenesis due to favorable resistance characteristics. Selection experiments, followed by either targeted or genome-wide next-generation-sequencing-based Tn insertion sit

SUBMITTER: Lennen RM 

PROVIDER: S-EPMC4178669 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature