Deep sequencing of norovirus genomes defines evolutionary patterns in an urban tropical setting.

ABSTRACT:

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Norovirus is a highly transmissible infectious agent that causes epidemic gastroenteritis in susceptible children and adults. Norovirus infections can be severe and can be initiated from an exceptionally small number of viral particles. Detailed genome sequence data are useful for tracking norovirus transmission and evolution. To address this need, we have developed a whole-genome deep-sequencing method that generates entire genome sequences from small amounts of clinical specimens. This novel approach employs an algorithm for reverse transcription and PCR amplification primer design using all of the publically available norovirus sequence data. Deep sequencing and de novo assembly were used to generate norovirus genomes from a large set of diarrheal patients attending t

SUBMITTER: Cotten M 

PROVIDER: S-EPMC4178781 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature