Up to four distinct polypeptides are produced from the γ34.5 open reading frame of herpes simplex virus 2.

ABSTRACT:

Unlabelled

The herpes simplex virus 1 (HSV-1) ICP34.5 protein strongly influences neurovirulence and regulates several cellular antiviral responses. Despite the clinical importance of HSV-2, relatively little is known about its ICP34.5 ortholog. We found that HSV-2 produces up to four distinct forms of ICP34.5 in infected cells: a full-length protein, one shorter form sharing the N terminus, and two shorter forms sharing the C terminus. These forms appeared with similar kinetics and accumulated in cells over much of the replication cycle. We confirmed that the N-terminal form is translated from the primary unspliced transcript to a stop codon within the intron unique to HSV-2 γ34.5. We found that the N-terminal form was produced in a variety of cell types and by 9 of 10 clinical is

SUBMITTER: Korom M 

PROVIDER: S-EPMC4178799 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature