Three-dimensional imaging of HIV-1 virological synapses reveals membrane architectures involved in virus transmission.

ABSTRACT:

Unlabelled

HIV transmission efficiency is greatly increased when viruses are transmitted at virological synapses formed between infected and uninfected cells. We have previously shown that virological synapses formed between HIV-pulsed mature dendritic cells (DCs) and uninfected T cells contain interdigitated membrane surfaces, with T cell filopodia extending toward virions sequestered deep inside invaginations formed on the DC membrane. To explore membrane structural changes relevant to HIV transmission across other types of intercellular conjugates, we used a combination of light and focused ion beam scanning electron microscopy (FIB-SEM) to determine the three-dimensional (3D) architectures of contact regions between HIV-1-infected CD4(+) T cells and either uninfected human CD4(

SUBMITTER: Do T 

PROVIDER: S-EPMC4178837 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature