Modulation of stop codon read-through efficiency and its effect on the replication of murine leukemia virus.

ABSTRACT:

Unlabelled

Translational readthrough--suppression of termination at a stop codon--is exploited in the replication cycles of several viruses and represents a potential target for antiviral intervention. In the gammaretroviruses, typified by Moloney murine leukemia virus (MuLV), gag and pol are in the same reading frame, separated by a UAG stop codon, and termination codon readthrough is required for expression of the viral Gag-Pol fusion protein. Here, we investigated the effect on MuLV replication of modulating readthrough efficiency. We began by manipulating the readthrough signal in the context of an infectious viral clone to generate a series of MuLV variants in which readthrough was stimulated or reduced. In carefully controlled infectivity assays, it was found that reducing th

SUBMITTER: Csibra E 

PROVIDER: S-EPMC4178896 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature