Colorimetric detection of Ehrlichia canis via nucleic acid hybridization in gold nano-colloids.

ABSTRACT: Canine monocytic ehrlichiosis (CME) is a major thick-bone disease of dog caused by Ehrlichia canis. Detection of this causal agent outside the laboratory using conventional methods is not effective enough. Thus an assay for E. canis detection based on the p30 outer membrane protein gene was developed. It was based on the p30 gene amplification using loop-mediated isothermal DNA amplification (LAMP). The primer set specific to six areas within the target gene were designed and tested for their sensitivity and specificity. Detection of DNA signals was based on modulation of gold nanoparticles' surface properties and performing DNA/DNA hybridization using an oligonucleotide probe. Presence of target DNA affected the gold colloid nanoparticles in terms of particle aggregation with a plasmonic

SUBMITTER: Muangchuen A 

PROVIDER: S-EPMC4178980 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature