An open-source analytical platform for analysis of C. elegans swimming-induced paralysis.

ABSTRACT:

Background

The nematode Caenhorhabditis elegans offers great power for the identification and characterization of genes that regulate behavior. In support of this effort, analytical methods are required that provide dimensional analyses of subcomponents of behavior. Previously, we demonstrated that loss of the presynaptic dopamine (DA) transporter, dat-1, evokes DA-dependent Swimming-Induced Paralysis (Swip) (Mcdonald et al., 2007), a behavior compatible with forward genetic screens (Hardaway et al., 2012).

New method

Here, we detail the development and implementation of SwimR, a set of tools that provide for an automated, kinetic analysis of C. elegans Swip. SwimR relies on open source programs that can be freely implemented and modified.

Results

We show that SwimR c

SUBMITTER: Hardaway JA 

PROVIDER: S-EPMC4179448 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature