Comparative proteomic analysis of renal tissue in IgA nephropathy with iTRAQ quantitative proteomics.

ABSTRACT: Immunoglobulin (Ig) A nephropathy (IgAN) is the most common form of glomerulonephritis. In clinical practice, it is difficult to monitor the repeating relapse in patients suffering from IgAN, which usually occurs within 10 years of end-stage renal disease. In order to identify and quantify the total protein content in the renal tissue of patients with IgAN, isobaric tags for relative and absolute quantification (iTRAQ) technology was performed. iTRAQ coupled with multiple chromatographic fractionation and tandem mass spectrometry was used to analyze the total protein of normal renal tissue in IgAN and healthy patients. The individual proteins were identified by the Mascot search engine and any that were differentially expressed were monitored. A total of 574 different proteins were identif

SUBMITTER: Sui W 

PROVIDER: S-EPMC4179707 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature