Vertical transmission of highly similar bla CTX-M-1-harboring IncI1 plasmids in Escherichia coli with different MLST types in the poultry production pyramid.

ABSTRACT:

Objectives

The purpose of this study was to characterize sets of extended-spectrum β-lactamases (ESBL)-producing Enterobacteriaceae collected longitudinally from different flocks of broiler breeders, meconium of 1-day-old broilers from theses breeder flocks, as well as from these broiler flocks before slaughter.

Methods

Five sets of ESBL-producing Escherichia coli were studied by multi-locus sequence typing (MLST), phylogenetic grouping, PCR-based replicon typing and resistance profiling. The bla CTX-M-1-harboring plasmids of one set (pHV295.1, pHV114.1, and pHV292.1) were fully sequenced and subjected to comparative analysis.

Results

Eleven different MLST sequence types (ST) were identified with ST1056 the predominant one, isolated in all five sets either on the broi

SUBMITTER: Zurfluh K 

PROVIDER: S-EPMC4179741 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature