Label-free LC-MSe in tissue and serum reveals protein networks underlying differences between benign and malignant serous ovarian tumors.

ABSTRACT:

Purpose

To identify proteins and (molecular/biological) pathways associated with differences between benign and malignant epithelial ovarian tumors.

Experimental procedures

Serum of six patients with a serous adenocarcinoma of the ovary was collected before treatment, with a control group consisting of six matched patients with a serous cystadenoma. In addition to the serum, homogeneous regions of cells exhibiting uniform histology were isolated from benign and cancerous tissue by laser microdissection. We subsequently employed label-free liquid chromatography tandem mass spectrometry (LC-MSe) to identify proteins in these serum and tissues samples. Analyses of differential expression between samples were performed using Bioconductor packages and in-house scripts in the stat

SUBMITTER: Wegdam W 

PROVIDER: S-EPMC4180266 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature