MicroRNA expression differentiates squamous epithelium from Barrett's esophagus and esophageal cancer.

ABSTRACT:

Background

Current strategies fail to identify most patients with esophageal adenocarcinoma (EAC) before the disease becomes advanced and incurable. Given the dismal prognosis associated with EAC, improvements in detection of early-stage esophageal neoplasia are needed.

Aim

We sought to assess whether differential expression of microRNAs could discriminate between squamous epithelium, Barrett's esophagus (BE), and EAC.

Methods

We analyzed microRNA expression in a discovery cohort of human endoscopic biopsy samples from 36 patients representing normal squamous esophagus (n = 11), BE (n = 14), and high-grade dysplasia/EAC (n = 11). RNA was assessed using microarrays representing 847 human microRNAs followed by quantitative real-time polymerase chain reaction (qRT-PCR) v

SUBMITTER: Garman KS 

PROVIDER: S-EPMC4180409 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature