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Multiplexed aberration measurement for deep tissue imaging in vivo.

ABSTRACT: We describe an adaptive optics method that modulates the intensity or phase of light rays at multiple pupil segments in parallel to determine the sample-induced aberration. Applicable to fluorescent protein-labeled structures of arbitrary complexity, it allowed us to obtain diffraction-limited resolution in various samples in vivo. For the strongly scattering mouse brain, a single aberration correction improved structural and functional imaging of fine neuronal processes over a large imaging volume.

SUBMITTER: Wang C 

PROVIDER: S-EPMC4180771 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature

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Multiplexed aberration measurement for deep tissue imaging in vivo.

Wang Chen C   Liu Rui R   Milkie Daniel E DE   Sun Wenzhi W   Tan Zhongchao Z   Kerlin Aaron A   Chen Tsai-Wen TW   Kim Douglas S DS   Ji Na N  

Nature methods 20140817 10

We describe an adaptive optics method that modulates the intensity or phase of light rays at multiple pupil segments in parallel to determine the sample-induced aberration. Applicable to fluorescent protein-labeled structures of arbitrary complexity, it allowed us to obtain diffraction-limited resolution in various samples in vivo. For the strongly scattering mouse brain, a single aberration correction improved structural and functional imaging of fine neuronal processes over a large imaging vol  ...[more]