A high-concentrate diet induced colonic epithelial barrier disruption is associated with the activating of cell apoptosis in lactating goats.

ABSTRACT:

Background

In ruminants, lower ruminal pH causes massive disruption of ruminal epithelial structure during periods of feeding high-concentrate diets. However, the influence of excessive organic fatty acids in the lumen of hindgut on the epithelial structure is unclear. In this study, twelve mid-lactating goats were randomly assigned to either a HC diet group (65% concentrate of dry matter; n = 6) or a LC diet group (35% concentrate of dry matter; n = 6) for 10 weeks. The colonic epithelial structure was detected by HE staining and transmission electron microscopy (TEM), and the apoptotic status of epithelial cells was estimated by TUNEL method and caspase activities.

Results

HC goats showed higher level of free lipopolysaccharide (LPS) in rumen fluid (p < 0.01) but not in co

SUBMITTER: Tao S 

PROVIDER: S-EPMC4180839 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature