Detection of Toxoplasma gondii oocysts in soils in northwestern China using a new semi-nested PCR assay.

ABSTRACT:

Background

Toxoplasma gondii is a zoonotic pathogen that can infect a range of animals and humans. Ingestion of T. gondii oocysts in soil is a significant transmission route for humans and animals acquiring toxoplasmosis. In the present study, we developed a new semi-nested PCR method to determine T. gondii oocysts distribution in soils in northwestern China.

Results

The one tube semi-nested PCR assay was developed to detect the oocysts of T. gondii in soil, targeting the repetitive 529 bp fragment of T. gondii genomic DNA. Then a total of 268 soil samples, including 148 samples from Gansu Province and 120 samples from Qinghai Province, northwestern China, were examined by the semi-nested PCR method. One third of the positive samples were sequenced. The sensitivity of the se

SUBMITTER: Wang M 

PROVIDER: S-EPMC4181410 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature