Deep sequencing of the X chromosome reveals the proliferation history of colorectal adenomas.

ABSTRACT:

Background

Mismatch repair deficient colorectal adenomas are composed of transformed cells that descend from a common founder and progressively accumulate genomic alterations. The proliferation history of these tumors is still largely unknown. Here we present a novel approach to rebuild the proliferation trees that recapitulate the history of individual colorectal adenomas by mapping the progressive acquisition of somatic point mutations during tumor growth.

Results

Using our approach, we called high and low frequency mutations acquired in the X chromosome of four mismatch repair deficient colorectal adenomas deriving from male individuals. We clustered these mutations according to their frequencies and rebuilt the proliferation trees directly from the mutation clusters usin

SUBMITTER: De Grassi A 

PROVIDER: S-EPMC4181412 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature