Purification of the cystic fibrosis transmembrane conductance regulator protein expressed in Saccharomyces cerevisiae.

ABSTRACT: Defects in the cystic fibrosis transmembrane conductance regulator (CFTR) protein cause cystic fibrosis (CF), an autosomal recessive disease that currently limits the average life expectancy of sufferers to <40 years of age. The development of novel drug molecules to restore the activity of CFTR is an important goal in the treatment CF, and the isolation of functionally active CFTR is a useful step towards achieving this goal. We describe two methods for the purification of CFTR from a eukaryotic heterologous expression system, S. cerevisiae. Like prokaryotic systems, S. cerevisiae can be rapidly grown in the lab at low cost, but can also traffic and posttranslationally modify large membrane proteins. The selection of detergents for solubilization and purification is a critical step in the

SUBMITTER: Pollock N 

PROVIDER: S-EPMC4181556 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature