Chimeric antibodies with extended half-life in ferrets.

ABSTRACT:

Background

Ferrets have long been used as a disease model for the study of influenza vaccines, but a more recent use has been for the study of human monoclonal antibodies directed against influenza viruses. Published data suggest that human antibodies are cleared unusually quickly from the ferret and that immune responses may be partially responsible. This immunogenicity increases variability within groups and may present an obstacle to long-term studies.

Objective

Our aim was to identify an antibody design with reduced immunogenicity and longer circulating half-life in ferrets.

Methods

The constant region coding sequences for ferret immunoglobulin G were cloned, and chimeric human/ferret antibodies were expressed and purified. Some of the chimeric antibodies included

SUBMITTER: Nesspor TC 

PROVIDER: S-EPMC4181826 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature