Diversity of the antibody response to tetanus toxoid: comparison of hybridoma library to phage display library.

ABSTRACT: Monoclonal antibodies are important tools in research and since the 1990s have been an important therapeutic class targeting a wide variety of diseases. Earlier methods of mAb production relied exclusively on the lengthy process of making hybridomas. The advent of phage display technology introduced an alternative approach for mAb production. A potential concern with this approach is its complete dependence on an in vitro selection process, which may result in selection of V(H)-V(L) pairs normally eliminated during the in vivo selection process. The diversity of V(H)-V(L) pairs selected from phage display libraries relative to an endogenous response is unknown. To address these questions, we constructed a panel of hybridomas and a phage display library using the spleen of a single tetanus

SUBMITTER: Sorouri M 

PROVIDER: S-EPMC4182348 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature