Proteomic study reveals a functional network of cancer markers in the G1-Stage of the breast cancer cell cycle.

ABSTRACT:

Background

Cancer cells are characterized by a deregulated cell cycle that facilitates abnormal proliferation by allowing cells to by-pass tightly regulated molecular checkpoints such as the G1/S restriction point. To facilitate early diagnosis and the identification of new drug targets, current research efforts focus on studies that could lead to the development of protein panels that collectively can improve the effectiveness of our response to the detection of a life-threatening disease.

Methods

Estrogen-responsive MCF-7 cells were cultured and arrested by serum deprivation in the G1-stage of the cell cycle, and fractionated into nuclear and cytoplasmic fractions. The protein extracts were trypsinized and analyzed by liquid chromatography--mass spectrometry (MS), and the

SUBMITTER: Tenga MJ 

PROVIDER: S-EPMC4182858 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature