High dose ionizing radiation regulates micro RNA and gene expression changes in human peripheral blood mononuclear cells.

ABSTRACT:

Background

High dose ionizing radiation (IR) induces potent toxic cell effects mediated by either direct DNA damage or the production of reactive oxygen species (ROS). IR-induced modulations in multiple biological processes have been proposed to be partly regulated by radiosensitive microRNA (miRNA). In order to gain new insights into the role of miRNAs in the regulation of biological processes after IR, we have investigated changes in mRNA and miRNA expression after high dose IR.

Results

IR induced changes in the mRNA and miRNA profiles of human peripheral blood mononuclear cells (PBMCs). When comparing non-irradiated and irradiated samples, we detected a time-dependent increase in differentially expressed mRNAs and miRNAs, with the highest differences detectable 20 hours a

SUBMITTER: Beer L 

PROVIDER: S-EPMC4182888 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature