Spatial control of microtubule length and lifetime by opposing stabilizing and destabilizing functions of Kinesin-8.

ABSTRACT:

Background

To function in diverse cellular processes, the dynamic behavior of microtubules (MTs) must be differentially regulated within the cell. In budding yeast, the spindle position checkpoint (SPOC) inhibits mitotic exit in response to mispositioned spindles. To maintain SPOC-mediated anaphase arrest, astral MTs must maintain persistent interactions with and/or extend through the bud neck. However, the molecular mechanisms that ensure the stability of these interactions are not known.

Results

The presence of an MT extending through and/or interacting with the bud neck is maintained by spatial control of catastrophe and rescue, which extends MT lifetime >25-fold and controls the length of dynamic MTs within the bud compartment. Moreover, the single kinesin-8 motor Kip3 a

SUBMITTER: Fukuda Y 

PROVIDER: S-EPMC4182928 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature