Cycloheximide treatment to identify components of the transitional transcriptome in PACAP-induced PC12 cell differentiation.

ABSTRACT: Pituitary adenylate cyclase-activating polypeptide (PACAP) promotes neurite outgrowth, reduces proliferation and inhibits apoptosis of PC12 cells. We have partially characterized the transcriptome changes induced by PACAP after 6 h of treatment, when commitment to differentiation has occurred. Here, we have investigated the effects of a 6-h treatment with PACAP (10(-7) m) in the presence of cycloheximide (5 microm) to identify, via superinduction, components of the transitional transcriptome initially induced by PACAP and potentially participating in the regulation of late-response genes required for differentiation. Approximately 100 new transcripts were identified in this screen, i.e. as many individual genes as make up the 6-h PACAP differentiation transcriptome itself. Six known transc

SUBMITTER: Ravni A 

PROVIDER: S-EPMC4183198 | biostudies-literature | 2006 Aug

REPOSITORIES: biostudies-literature