Sources and distribution of surface water fecal contamination and prevalence of schistosomiasis in a Brazilian village.

ABSTRACT:

Background

The relationship between poor sanitation and the parasitic infection schistosomiasis is well-known, but still rarely investigated directly and quantitatively. In a Brazilian village we correlated the spatial concentration of human fecal contamination of its main river and the prevalence of schistosomiasis.

Methods

We validated three bacterial markers of contamination in this population by high throughput sequencing of the 16S rRNA gene and qPCR of feces from local residents. The qPCR of genetic markers from the 16S rRNA gene of Bacteroides-Prevotella group, Bacteroides HF8 cluster, and Lachnospiraceae Lachno2 cluster as well as sequencing was performed on georeferenced samples of river water. Ninety-six percent of residents were examined for schistosomiasis.

Fi

SUBMITTER: Ponce-Terashima R 

PROVIDER: S-EPMC4183440 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature