Disease detection by ultrasensitive quantification of microdosed synthetic urinary biomarkers.

ABSTRACT: The delivery of exogenous agents can enable noninvasive disease monitoring, but existing low-dose approaches require complex infrastructure. In this paper, we describe a microdose-scale injectable formulation of nanoparticles that interrogate the activity of thrombin, a key regulator of clotting, and produce urinary reporters of disease state. We establish a customized single molecule detection assay that enables urinary discrimination of thromboembolic disease in mice using doses of the nanoparticulate diagnostic agents that fall under regulatory guidelines for "microdosing."

SUBMITTER: Warren AD 

PROVIDER: S-EPMC4183649 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature