Structural mass spectrometry of tissue extracts to distinguish cancerous and non-cancerous breast diseases.

ABSTRACT: Aberrant metabolism in breast cancer tumors has been widely studied by both targeted and untargeted analyses to characterize the affected metabolic pathways. In this work, we utilize ultra-performance liquid chromatography (UPLC) in tandem with ion mobility-mass spectrometry (IM-MS), which provides chromatographic, structural, and mass information, to characterize the aberrant metabolism associated with breast diseases such as cancer. In a double-blind analysis of matched control (n = 3) and disease tissues (n = 3), samples were homogenized, polar metabolites were extracted, and the extracts were characterized by UPLC-IM-MS/MS. Principle component analysis revealed a strong separation between disease tissues, with one diseased tissue clustering with the control tissues along PC1 and two ot

SUBMITTER: Hines KM 

PROVIDER: S-EPMC4183709 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature