Chromosome 9p deletion in clear cell renal cell carcinoma predicts recurrence and survival following surgery.

ABSTRACT:

Background

Wider clinical applications of 9p status in clear cell renal cell carcinoma (ccRCC) are limited owing to the lack of validation and consensus for interphase fluorescent in situ hybridisation (I-FISH) scoring technique. The aim of this study was to analytically validate the applicability of I-FISH in assessing 9p deletion in ccRCC and to clinically assess its long-term prognostic impact following surgical excision of ccRCC.

Methods

Tissue microarrays were constructed from 108 renal cell carcinoma (RCC) tumour paraffin blocks. Interphase fluorescent in situ hybridisation analysis was undertaken based on preset criteria by two independent observers to assess interobserver variability. 9p status in ccRCC tumours was determined and correlated to clinicopathological var

SUBMITTER: El-Mokadem I 

PROVIDER: S-EPMC4183850 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature