Exploring the functional soil-microbe interface and exoenzymes through soil metaexoproteomics.

ABSTRACT: Functionally important proteins at the interface of cell and soil are of potentially low abundance when compared with commonly recovered intracellular proteins. A novel approach was developed and used to extract the metaexoproteome, the subset of proteins found outside the cell, in the context of a soil enriched with the nitrogen-containing recalcitrant polymer chitin. The majority of proteins recovered was of bacterial origin and localized to the outer membrane or extracellular milieu. A wide variety of transporter proteins were identified, particularly those associated with amino-acid and phosphate uptake. The metaexoproteome extract retained chitinolytic activity and we were successful in detecting Nocardiopsis-like chitinases that correlated with the glycoside hydrolase family 18 (GH18

SUBMITTER: Johnson-Rollings AS 

PROVIDER: S-EPMC4184004 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature