Muscle on a chip: in vitro contractility assays for smooth and striated muscle.

ABSTRACT:

Introduction

To evaluate the viability of a muscle tissue, it is essential to measure the tissue's contractile performance as well as to control its structure. Accurate contractility data can aid in development of more effective and safer drugs. This can be accomplished with a robust in vitro contractility assay applicable to various types of muscle tissue.

Methods

The devices developed in this work were based on the muscular thin film (MTF) technology, in which an elastic film is manufactured with a 2D engineered muscle tissue on one side. The tissue template is made by patterning extracellular matrix with microcontact printing. When muscle cells are seeded on the film, they self-organize with respect to the geometric cues in the matrix to form a tissue.

Results

Seve

SUBMITTER: Grosberg A 

PROVIDER: S-EPMC4184427 | biostudies-literature | 2012 May-Jun

REPOSITORIES: biostudies-literature