Undressing of Waddlia chondrophila to enrich its outer membrane proteins to develop a new species-specific ELISA.

ABSTRACT: Waddlia chondrophila, an obligate intracellular bacterium of the Chlamydiales order, is considered as an agent of bovine abortion and a likely cause of miscarriage in humans. Its role in respiratory diseases was questioned after the detection of its DNA in clinical samples taken from patients suffering from pneumonia or bronchiolitis. To better define the role of Waddlia in both miscarriage and pneumonia, a tool allowing large-scale serological investigations of Waddlia seropositivity is needed. Therefore, enriched outer membrane proteins of W. chondrophila were used as antigens to develop a specific ELISA. After thorough analytical optimization, the ELISA was validated by comparison with micro-immunofluorescence and it showed a sensitivity above 85% with 100% specificity. The ELISA was su

SUBMITTER: Lienard J 

PROVIDER: S-EPMC4184618 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature