Integrative DNA methylation and gene expression analysis identifies discoidin domain receptor 1 association with idiopathic nonobstructive azoospermia.

ABSTRACT:

Objective

To evaluate the association between promoter DNA methylation and discoidin domain receptor 1 (DDR1) gene expression in men with nonobstructive azoospermia (NOA).

Design

Comparing fibroblasts cultured from testicular biopsies using a high resolution Infinium 450K methylation array.

Setting

Basic research laboratory.

Patient(s)

Men with NOA (n = 16) and with normal spermatogenesis (n = 5).

Intervention(s)

None.

Main outcome measure(s)

Bisulfite clonal sequencing for validation and quantification of CpG methylation of DDR1; gene expression analysis of DDR1 with quantitative polymerase chain reaction and immunohistochemistry to validate the array results at mRNA and protein levels.

Result(s)

We validated promoter methylation, mRNA and

SUBMITTER: Ramasamy R 

PROVIDER: S-EPMC4184939 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature