Genetically encoded unstrained olefins for live cell labeling with tetrazine dyes.

ABSTRACT: A number of non-canonical amino acids (NCAAs) with unstrained olefins are genetically encoded using mutant pyrrolysyl-tRNA synthetase-tRNA(Pyl)(CUA) pairs. These NCAAs readily undergo inverse electron-demand Diels-Alder cycloadditions with tetrazine dyes, leading to selective labeling of proteins bearing these NCAAs in live cells.

SUBMITTER: Lee YJ 

PROVIDER: S-EPMC4184966 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature