Fatty acid composition and desaturase gene expression in flax (Linum usitatissimum L.).

ABSTRACT: Little is known about the relationship between expression levels of fatty acid desaturase genes during seed development and fatty acid (FA) composition in flax. In the present study, we looked at promoter structural variations of six FA desaturase genes and their relative expression throughout seed development. Computational analysis of the nucleotide sequences of the sad1, sad2, fad2a, fad2b, fad3a and fad3b promoters showed several basic transcriptional elements including CAAT and TATA boxes, and several putative target-binding sites for transcription factors, which have been reported to be involved in the regulation of lipid metabolism. Using semi-quantitative reverse transcriptase PCR, the expression patterns throughout seed development of the six FA desaturase genes were measured in s

SUBMITTER: Thambugala D 

PROVIDER: S-EPMC4185102 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature