In vivo microscopy reveals extensive embedding of capillaries within the sarcolemma of skeletal muscle fibers.

ABSTRACT:

Objective

To provide insight into mitochondrial function in vivo, we evaluated the 3D spatial relationship between capillaries, mitochondria, and muscle fibers in live mice.

Methods

3D volumes of in vivo murine TA muscles were imaged by MPM. Muscle fiber type, mitochondrial distribution, number of capillaries, and capillary-to-fiber contact were assessed. The role of Mb-facilitated diffusion was examined in Mb KO mice. Distribution of GLUT4 was also evaluated in the context of the capillary and mitochondrial network.

Results

MPM revealed that 43.6 ± 3.3% of oxidative fiber capillaries had ≥50% of their circumference embedded in a groove in the sarcolemma, in vivo. Embedded capillaries were tightly associated with dense mitochondrial populations lateral to capillary gr

SUBMITTER: Glancy B 

PROVIDER: S-EPMC4185432 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature