A polymerase chain reaction-based method for isolating clones from a complimentary DNA library in sheep.

ABSTRACT: The sheep (Ovis aries) is favored by many musculoskeletal tissue engineering groups as a large animal model because of its docile temperament and ease of husbandry. The size and weight of sheep are comparable to humans, which allows for the use of implants and fixation devices used in human clinical practice. The construction of a complimentary DNA (cDNA) library can capture the expression of genes in both a tissue- and time-specific manner. cDNA libraries have been a consistent source of gene discovery ever since the technology became commonplace more than three decades ago. Here, we describe the construction of a cDNA library using cells derived from sheep bones based on the pBluescript cDNA kit. Thirty clones were picked at random and sequenced. This led to the identification of a novel

SUBMITTER: Friis TE 

PROVIDER: S-EPMC4186646 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature