Exploring the potential of next-generation sequencing in detection of respiratory viruses.

ABSTRACT: Efficient detection of human respiratory viral pathogens is crucial in the management of patients with acute respiratory tract infection. Sequence-independent amplification of nucleic acids combined with next-generation sequencing technology and bioinformatics analyses is a promising strategy for identifying pathogens in clinical and public health settings. It allows the characterization of hundreds of different known pathogens simultaneously and of novel pathogens that elude conventional testing. However, major hurdles for its routine use exist, including cost, turnaround time, and especially sensitivity of the assay, as the detection limit is dependent on viral load, host genetic material, and sequencing depth. To obtain insights into these aspects, we analyzed nasopharyngeal aspirates f

SUBMITTER: Prachayangprecha S 

PROVIDER: S-EPMC4187785 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature