Identification and characterization of outer membrane vesicle-associated proteins in Salmonella enterica serovar Typhimurium.

ABSTRACT: Salmonella enterica serovar Typhimurium is a primary cause of enteric diseases and has acquired a variety of virulence factors during its evolution into a pathogen. Secreted virulence factors interact with commensal flora and host cells and enable Salmonella to survive and thrive in hostile environments. Outer membrane vesicles (OMVs) released from many Gram-negative bacteria function as a mechanism for the secretion of complex mixtures, including virulence factors. We performed a proteomic analysis of OMVs that were isolated under standard laboratory and acidic minimal medium conditions and identified 14 OMV-associated proteins that were observed in the OMV fraction isolated only under the acidic minimal medium conditions, which reproduced the nutrient-deficient intracellular milieu. The

SUBMITTER: Bai J 

PROVIDER: S-EPMC4187864 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature